Sartorius, VS-AVPQ502, Vivapure AdenoPACK 500 RT, Kit for Fast Purification
Includes:
- Sartobind Q 75 unit with protective end caps
- 2 x Pump tube set (Luer connector fits both Sartobind Q 75 and Sartopore units)
- 1 x Sartopore 2 150 clarifying filter
- 1 x Benzonase Nuclease stock (12.5 U/µl)
- 60 ml Loading Buffer (10x)
- 30 ml Washing Buffer (10x)
- 20 ml Elution Buffer
- 10 ml syringe
- 2 x Vivaspin 20 concentrators with 100 kDa MWCO PES membrane
Complete kit for fast purification of up to 3 x 1013 adenovirus type 5 particles from 500 ml cell culture. Contains 1 AdenoPACK 500 syringe filter unit with Sartobind ion exchange membrane, to be operated best by a laboratory pump, plus all buffers and further accessories. Vivapure AdenoPACK 500 is the direct upscale kit to the AdenoPACK 100, for adenovirus purification. In only 2 hours up to 3 x 1013 adenovirus particles are purified and concentrated from 500 ml cell culture. This completely ready-to-use kit is conveniently operated by a laboratory pump, offering optimal flow control and minimal hands-on time. This easy to use product replaces lengthy and inefficient caesium chloride density gradient methods.
Features:
- Pump driven kit
- Easy and controlled purification of Adenovirus from large cell culture volumes
- Virus purification based on membrane adsorber technology
- High-speed process completed in about 2 hours
- Convenient, easy to use alternative to CsCl density gradients
- Able to process 10 to 25 plates (up to 500 ml)
